| Abstract: | 近年來，人們飲食習慣的改變，伴隨著許多代謝相關的慢性疾病產生，過去已有報告指出，肥胖、第二型糖尿病及非酒精性脂肪肝都與肝臟代謝異常相關。本研究主要針對肝代謝綜合症進行探討。於此研究中，我們以30%高果糖玉米糖漿及60%高脂質飼糧誘導肝代謝綜合症，誘導小鼠額外給予 120、360、600 mg/kg/day 之葡萄皮萃取物十週。測定血清中天門冬胺酸轉胺酶、丙胺酸轉胺酶、三酸甘油酯、總膽固醇及發炎相關細胞激素，利用油紅染色、H&E染色及TLR-4、4-HNE免疫組織化學染色來檢測脂肪堆積。結果顯示，葡萄皮萃取物可以減少血清中天門冬胺酸轉胺酶、丙胺酸轉胺酶、三酸甘油酯、總膽固醇。葡萄皮萃取物也可以調節IL-1β、IL-6、TNF-α、MCP-1及TSLP等細胞激素在肝臟中含量。另外，在油紅染色及H&E染色結果觀察到，葡萄皮萃取物可以減少脂肪的堆積。根據以上結果，葡萄皮萃取物在肝代謝綜合症中具有改善脂肪堆積和炎症反應之作用。(關鍵字：細胞激素、炎症反應、肝代謝綜合症、葡萄皮萃取物)
In recent years, changes in people's dietary, along with many metabolic-related chronic diseases. This study aimed to explore the hepatic manifestations of the metabolic syndrome (HMMS) including obesity, type II diabetes and non-alcoholic fatty liver disease. In this study, we want to evaluate of grape skin extract against the onset of HMMS induced by 60% high-fat diet and drinking with 30% high-fructose corn syrup solution. Mice administered with grape skin extract (120, 360 and 600 mg/kg/day) for 10 weeks. Serum aspartate aminotransferase (sAST) alanine aminotransferase (sALT), triglyceride (sTG) and total cholesterol (sTC) and inflammatory cytokines were determined. Lipid accumulation in liver tissue sections were determinate by Oil-Red O staining, H&E staining and immunohistochemical staining for toll-like receptor 4 (TLR-4), 4-hydroxy-trans-2-nonenal, 4-hydroxynonenal (4-HNE). Our result indicated grape skin extract could reduce sALT, sTG and sTC. Furthermore, grape skin extract could regutate hepatic interleukin-1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and thymic stromal lymphopoietin (TSLP). In addition, the histology stained by Oil-Red O and H&E revealed grape skin extract decreased lipid accumulation. According to these results, it is suggested that grape skin extract could improve lipid accumulation in and inflammatory in HMMS. (Keywords: Cytokine, Inflammation, Hepatic manifestations of the metabolic syndrome, Grape skin extract) |
