本研究期利用省產極為豐富且廉價之豬皮製成膠原蛋白粉末（ collagen powder ）後，運用酵素水解膠原蛋白以製成富含絲胺酸（ serine）、甘 胺酸（glycine ）、丙胺酸（alanine ）、酪胺酸（ tyrosine）等游離 胺基酸和可溶性▋（soluble peptide ）之產品。其結果顯示：一、經過 不同程度加熱之膠原蛋白，其隨加熱程度愈大，無花果▋（ficin ）水解 膠原蛋白之效果有增強的趨勢，但木瓜▋（papain）與胃蛋白▋（pepsin ）則反之。另無花果▋可解出較多量之游離態目標胺基酸，而胃蛋白▋可 水解出較多量存在於可溶性中的目標胺基酸。二、由酵素濃度和反應時間 之影響顯示，胃蛋白▋及無花果▋分別於15及20％酵素濃度並水解48hrs 均有較佳之水解效果，而於整體考慮後，本研究係以20％無花果▋水解 48hrs 為製造豬皮膠原蛋白水解物（collagen hydrolysate from pig skin）之最適條件。三、豬皮膠原蛋白水解物含3.13％絲胺酸、30.11％ 甘胺酸、 11.37％丙胺酸及2.66％酪胺酸，其有 15.57％為游離胺基酸， 餘者皆為可溶性▋（soluble peptide ）者，而這些▋態者在仿人體消化 實驗中有 81.67％可消化成游離態胺基酸。四、豬皮膠原蛋白水解物具吸 收紫外光能力，尤以吸收200∼300nm波長者最佳，而此波長範圍之紫外光 對皮膚之殺傷力最大，且隨水解物之濃度越高，吸紫外光能力越強。 The substance of this study was to use Taiwan''s plentiful and low-cost pig skin to extract the collagen powder and then use enzyme to hydrolyze the collagen to produce soluble peptides and free amino acids which contained serine,glycine, alanine and tyrosine.Therefore, using the collagen enzyme hydrolysate may produce a functional ingredient that bears the value of extensive use. The results showed that the ficin hydrolysis ability ascended with the increased heating period of collagen ; however, papain and pepsin had total contrarily effect. The ficin could hydrolyze more free target amino acids. The pepsin could hydrolyze more target amino acids insoluble peptide. Effects of using various enzyme concentrations and reaction time to hydrolyze collagen showed that 15 ％ pepsin or 20 ％ ficin hydrolyzed 48 hrs to have better hydrolysis effects. After entire consideration, the study chose an optimum that was 20 ％ ficin hydrolyzed 48hrs to produce the collagen hydrolysate of pig skin. The collagen hydrolysate from pig skin contained 3.13 ％ serine,30.11 ％ glycine, 11.37 ％ alanine and2.66 ％ tyrosine. The hydrolysate contained 15.57 ％ free amino acids, remainds were soluble peptide. In Vitrodigestion experiment showed that 81.67 ％ peptide amino acids could beeasily digested into free amino acids. The collagen hydrolysate from pig skin was also able to absorb U. V. light. The hydrolysate had a better absorbability of U.V. lightin wavelength ranged from 200 ∼ 300nm which could cause the most serious damage to people''s skin. Moreover, the U.V. light absorbability of hydrolysate ascended with its increased concentrations.
